Playable in all modes:
  • Stewie Griffin – Main Character
  • Brian Griffin – Main Character
Playable in Challenges and Multiplayer:
  • Peter Griffin
  • Lois Griffin
  • Chris Griffin
  • Meg Griffin
  • Quagmire
  • Cleveland
  • Mayor West
  • Death
Several members of the original cast from the show are present throughout the game to either help or entertain the player, but you will only have the option to play as the characters listed above.

Note: PC players have the option to use Peter or the Giant Chicken in the Chicken Fight! Level while playing in story mode.